Then we detected the NOD2 signaling pathway in podocytes and diabetic mice, our results showed that high glucose upregulated the expression of NOD2 as well as its downstream protein p-RIP2 and p-P65, resulting in the increase of inflammatory factors such as TNF-α, IL-6, IL-1β, and IL-18; in vivo, DKD mice had high expression of above proteins and inflammatory factors, our results were consistent with previous research that NOD2 is overexpressed in DKD. This evidence concerns the gene TNF and diabetic kidney disease.