At 60 days post‐infection, the spleen of OM‐TB‐TKO mice contained significantly lower frequencies of CD3+ CD4+ T cells (Figure 4C), CD69+ CD4+ T cells (Figure 4D), CD25+ CD69+ T cells (Figure 4F), PD1+ CD27− T cells (Figure 4I), and TIM3+ CD4+ T cells (Figure 4K), when compared against O‐TB‐TKO or Y‐TB‐TKO mice. This evidence concerns the gene CD4 and infection.