To investigate whether tRF-Cys-GCA-029-regulated PRKCG may play a role in BC cell malignancy and glycolysis metabolism, we synthesized PRKCG-overexpressing plasmid and then transfected it into MDA-MB-231 and MCF-7 cells to construct gain-of-function cell models (Fig. S6). Here, PRKCG is linked to breast cancer.