Therefore, in this study, we first combined single-cell RNA-Sequencing (scRNA-seq), proteomics, and bulk RNA-Sequencing (RNA-seq) in order to investigate UNC5A's expression, prognosis, associated pathways or drugs, and clinical or immune relevance in gliomas, laying the groundwork for UNC5A’s future related studies in gliomas. The gene discussed is UNC5A; the disease is central nervous system cancer.