In T. cruzi-induced acute myocarditis in mice, the introduction of blocking antibodies targeting PD-1 and PD-L1 resulted in an elevated presence of inflammatory infiltrates in the myocardium, accompanied by heightened levels of proinflammatory cytokines such as tumor necrosis factor α (TNF-α) and IFNγ. This evidence concerns the gene IFNG and myocarditis.