To further investigate whether 5-FU could more efficiently inhibit the proliferation of tumor cells with elevated PRDKC expression, we constructed a stable PRKDC-overexpressing HMCB cell line (PRKDC-OE-HMCB) using the pCDH-PRKDC-copGFP vector and knocked down PRKDC (PRKDC-KD-HMCB) utilizing pLKO.1-CMV-shPRKDC-copGFP. The gene discussed is PRKDC; the disease is neoplasm.