HMGB1 and neoplasm: Moreover, to validate the potential causal link of ROCK2 in promoting tumor metastasis through the phosphorylation of HMGB1, we also collected PDCs from patients (PDC_ROCK2Amp: patients harboring ROCK2 amplification, PDC_WT: patients without ROCK2 amplification) and further conducted proteomic and phosphoproteomic analysis (Fig. 5o).