PRKDC and neoplasm: Multivariate analysis of age, sex, histological type, etc., proteogenomic and phosphoproteomic analysis, combined with functional experiments utilizing both primary tumor cells derived from patients and in vitro assays revealed that PRKDC amplification not only led to increased cognate protein expression, but also strongly associated with the activation of one-carbon metabolism and, in turn, might promote tumor cell proliferation and impact prognosis.