EGFR and lung cancer: We also evaluated the sensitivity for in-depth mappingof phosphorylation sites in signaling pathways and druggable targets.The performance of SOP-Phos-DIA was further demonstrated in the mechanisticstudy of EGFR-TKI-resistant and sensitive lung cancer cell lines.With the use of commonly used reagents and instrumentation, efficientprocessing and high profiling sensitivity, this protocol can be easilyadapted for daily operation by nonexpert users in the general community.