The present study further analyzed Ki-67 expression at different cut-off values, a cut-off value of ≥ 50% was found to be the most effective in discriminating Ki-67 expression and was also identified as a reliable predictor of survival outcome in patients with DLBCL (three-year OS; 60% vs 34%; p = 0.026). This evidence concerns the gene MKI67 and diffuse large B-cell lymphoma.