The expression of LRP6, IRS1, PNPLA2, PPARA, PCK1, and FOXO1 was significantly (#p<0.05) reduced in T2D compared to ND controls, whereby LRP6 and IRS1 were the only targets passing adjustment for multiple testing (*q<0.05; ***q<0.001) (Figure 2C, Figure 2—figure supplement 1C). This evidence concerns the gene PNPLA2 and type 2 diabetes mellitus.