In this study, we constructed targeted microbubbles (TM) carrying anti‐VCAM‐1 polypeptide to explore their specific binding ability and target‐enhanced imaging ability during the development of AKI, investigate the changes of microcirculatory perfusion and inflammatory response in IRI‐AKI, and clarify the correlation of TM in ultrasound molecular imaging with serum and histopathological biomarkers of AKI. Here, VCAM1 is linked to acute kidney injury.