In addition, Western blot results showed that compared with control cells, in 293T cell line knockout of HDAC8 significantly reduced the protein expression of RIG-I, MAVS, p-TBK1 and p-IRF3 during VSV-GFP infection, which are key regulators of RLRs signaling pathway and subsequent immune response (Figure 3E). The gene discussed is RIGI; the disease is infection.