The second sample was taken when the MDS or PNH progressed to AML and phosphorylated Akt and Erk proteins were appeared in MDS patient (Figure 2 lane 9 and 10) and phosphorylated Akt were developed by PNH patient (Figure 2 lane 11 and 12) at the time of AML transformation. The gene discussed is AKT1; the disease is paroxysmal nocturnal hemoglobinuria.