When comparing clusters between patients with EoE before and after treatment and healthy subjects we found differences in size and intensity of the clusters for effector memory CD4+ T cells (cluster 1), central memory CD4+ T cells (cluster 2), memory T regs (cluster 3), and central memory CD8+ T cells (cluster 9) (Fig. 2A). This evidence concerns the gene CD8A and eosinophilic esophagitis.