To investigate the effect of MLT-Gel treatment on the levels of pro-inflammatory cytokines in the serum of RA mice, TNF-α, IL-12, IL-1β, Interferon-γ (IFN-γ), and Interleukin-17 A (IL-17 A) concentrations were measured using ELISA kits. The gene discussed is IL1B; the disease is rheumatoid arthritis.