In 23 patients (10.5% of the cohort, 22.3% of patients with variants) it was not possible to determine a pathogenic role of genetic variants found in PD genes; these cases included single previously unreported VUS in dominant genes (4 in LRRK2) and monoallelic variants in recessive genes (6 PRKN, 1 PINK1, 4 DJ1, 6 VPS13C, 3 ATP13A2, 1 DNAJC6, 2 PLA2G6, 50% likely pathogenic); 7 patients had multiple variants in more than one PD gene. This evidence concerns the gene PRKN and Parkinson disease.