The conclusion related to these three types of myeloid cells can be verified through immunofluorescence chromosomes to confirm the results of multi omics sequencing analysis: Macro_ ISG15, Macro_ NLRP3, Macro_ IER3, Mono_ CD14, Mono_ CD16 and cDC_ The expression level of LAMP3 increases in reactive tumor tissues, and these biomarkers may be one of the response mechanisms of pan cancer immunotherapy, and may also provide a basis for clinical monitoring and efficacy prediction. The gene discussed is ISG15; the disease is neoplasm.