To further prove the influence of complement on AKT2-dependent regulation of lysosomal function, we cultured iPSC-derived CFH (H/H) and (Y/Y) RPE cells with complement-competent human serum (CCHS), which provides complement factors including anaphylatoxins to mimic the age-induced increase in complement activation observed in AMD eyes29, and then treated cells with an AKT2 inhibitor22 and trehalose. Here, AKT2 is linked to age-related macular degeneration.