CD8A and neoplasm: Other changes to the tumor immune milieu upon treatment with EV-Ligand included increased expression of Granzyme in CD8+ T cells, upregulation of MHC II in DC subsets, increased M1 polarization of interstitial macrophages and enhanced expression of IFN-γ mRNA, all of which were significantly elevated compared with treatment with equivalent doses of free ligand treatments (Figures S10D and S10E).