However, exacerbated NAFLD in UCP1−/− mice fed with MCD was identified by increased lipid accumulation in the liver (Fig. 1F); increased serum AST and ALT as markers for NASH; and elevated levels of TG, total CHO (TC), and FFAs in the liver (Fig. 1G). The gene discussed is UCP1; the disease is metabolic dysfunction-associated steatohepatitis.