We demonstrate that CRISPR-Cas9–mediated genomic deletion of the NEAT1 PAS diminishes NEAT1_1 levels, which is accompanied by a significant increase in NEAT1_2 in GBM cells (Fig. 2), similar to previous studies which deleted the NEAT1 PAS in the human myelogenous leukemia haploid cell line HAP1 (29). Here, NEAT1 is linked to glioblastoma.