To further check whether GRA4 could degrade TBK1 during T. gondii cellular infection, we compared the intrinsic protein level change of both TBK1 and p‐TBK1 in macrophages infected with ME49wt and ME49Δgra4, and found ME49wt infection caused the degradation of TBK1, while knockout of GRA4 in ME49 rescued the protein reduction of TBK1 and enhanced TBK1 phosphorylation induced by T. gondii infection (Figure 3E; Figure S4G, Supporting Information). Here, TBK1 is linked to infection.