DDX53 and malignant colon neoplasm: We accomplished this by using an established mouse liver colon cancer metastatic model where the spleen is injected with CT26‐GFP colon cancer cells using a protocol we described elsewhere.[11] Mice received a Trp‐free diet for 3 days after intra‐splenic injection, followed by gavage with biotin labeled Trp (300 mg k−1g) 12 h prior to euthanasia. GFP+‐CT26 cells were sorted by FACS.