Using a 3D organoid model with KRAS mutant (MT) and KRAS wild type (WT) CRC cells, it was observed that glutamine deficiency curtailed the growth of both KRAS MT and KRAS WT cells and triggered an increase in GPER1 and asparagine synthase (ASNS) expression in KRAS MT cells. Here, GPER1 is linked to colorectal carcinoma.