In addition, SC79 enhanced the phosphorylation level of AKT, meanwhile, SC79 treatment restored AKT phosphorylation and CSC-like marker protein levels (Fig. 10A, Fig. S10A), CSC-like properties (Fig. 10B-G, Fig. S11A and B, Fig. S10B-G, Fig. S11C and D), EMT level (Fig. 10H, Fig. S10H), cell migration and invasion activity (Fig. 10I and J, Fig. S12A and B, Fig. S10I and J, Fig. S12C and D) and tumor metastasis in vivo (Fig. 10K) after TSP50 knockdown in breast cancer cells. The gene discussed is PRSS50; the disease is breast carcinoma.