To spatially confirm the co-expression of GZMA and IFN-γ in CD8+T cells and the expression of GSDMB in epithelial cells, we performed mIHC staining on both colon cancer and normal colon TMA (Fig. 2A: CK (green), CD8 (indigo), GZMA (yellow), GSDMB (purple) and IFN-γ (red)). The gene discussed is CD8A; the disease is colonic neoplasm.