Considering that PHF5A expression can affect OS of ACC, LIHC, LUAD, PAAD, READ and STAD and that TISCH online tool lacks datasets for ACC and READ, this study analyzed the association of PHF5A with TME in LIHC, NSCLC, PAAD and STAD at the single-cell sequencing level, which indicated that PHF5A was relevant to the TME in LIHC, NSCLC, PAAD and STAD and could be detected in malignant cells, immune cells and other cells (Fig. 4C). This evidence concerns the gene PHF5A and adrenal cortex carcinoma.