To finally investigate the effect of EGC-derived IL-6 on the SPP1+ TAM differentiation and tumor development in vivo, we orthotopically injected MC38 cells in the colonic mucosa of mice with a glial-specific deletion of IL-6 (SOX10CreERT2IL-6fl/fl) and their littermates (SOX10CreERT2IL-6wt/wt) (Fig. 3j). The gene discussed is SPP1; the disease is neoplasm.