SPP1 and colorectal carcinoma: By using the genes differentially expressed between SPP1+ TAMs and monocytes [data extracted from our single-cell RNA-seq (scRNAseq) murine orthotopic CRC dataset] as target genes, we prioritized candidate ligands derived from TME-CM EGCs (data extracted from bulk RNAseq in vitro CRC EGCs dataset) as potential drivers of this differentiation process (Fig. 3e).