We found that CyCMV/Flu vectors induced influenza-specific CD8+ and CD4 + TEM, with the influenza-specific CD8 + T cells being restricted by MHC-Ia in FL CyCMV/Flu-vaccinated MCM and MHC-II or MHC-E in dd CyCMV/Flu-vaccinated MCM. This evidence concerns the gene CD4 and influenza.