We conducted rescue studies in A549 and H1299 cells by transfecting with siControl or siUSP32 for 24 h, followed by transfecting with Flag or Flag-BAG3 to examine whether USP32 activates the RAF/MEK/ERK signaling cascade by increasing BAG3 expression in NSCLC cells. This evidence concerns the gene BAG3 and non-small cell lung carcinoma.