Accordingly, we conducted a functionaldomain-targeted CRISPR-Cas9 screen co-targeting paralog pairs of kinases,phosphatases, epigenetic regulators, and DNA binding proteins in three SCLC-Aand three SCLC-P cell lines (Figure 1A).Dependency scores (beta scores) for 4,341 single-gene and 4,387 double-geneknockouts were calculated using MAGeCK.26 Comparing beta scores between SCLC-A and SCLC-P celllines, we observed dramatic dependency differences for lineage TFs ASCL-1 andPOU2F3. This evidence concerns the gene ASCL1 and small cell lung carcinoma.