Interestingly, compared to chow-fed controls, hepatic Chrna2 dynamically responded to metabolic stress and was up-regulated approximately 4-fold in livers from mice challenged with a Gubra-Amylin non-alcoholic steatohepatitis diet (GAN diet), which has been shown to cause hepatic steatosis, inflammation, and fibrosis, similar to morphological characteristics in human MASH [22] (Figs 1F and S1C). Here, CHRNA2 is linked to fatty liver disease.