CHRNA2 and fatty liver disease: However, in response to HFD plus CCl4 treatment, Chatfl/fl;LysMCre mice exhibited worsened MASH-related parameters, including blood ALT levels, liver steatosis, collagen deposition, and hepatic gene expression levels for lipogenesis and injury, compared to controls, although differences between the 2 genotypes were not as profound as Chrna2-deficient animals relative to their corresponding controls (Fig 3S–3W, S7G and S7H).