In this study, we conducted a comprehensive assessment of the expression patterns of key EMT markers (N-cadherin, E-cadherin, Vimentin, SNAIL) and fibrosis-related markers (α-SMA, FN1, Col III) in a rat model with zinc deficiency using qPCR, WB analysis, and IHC. This evidence concerns the gene ACTA1 and Zinc deficiency.