Importantly, on the other hand, APO, UMB, and their combination protected rats from ACR-induced AKI by downregulating the NLRP3 inflammasome and inhibiting the assembly and activation of the NLRP3 inflammasome complex, thereby reducing the release of IL-1β and subsequent inflammation via modulating key components of the NLRP3 inflammasome pathway, such as NLRP3 itself, ASC, GSDMD, IL-1β, and caspase-1. This evidence concerns the gene AOPEP and acute kidney injury.