Areas annotated as having diffuse immune infiltration showed higher levels of MHCI (beta-2-microglobulin, B2M) and checkpoints IDO1, PD-L1, PD-1 and Tim-3 in tumor AOIs (Fig. 2d), and higher levels of T-cells (CD3) and Granzyme B in immune AOIs (Fig. 2e). Here, IDO1 is linked to neoplasm.