Out of 180 genes with data available in DepMap, 33 demonstrated essential roles in cell proliferation at a commonly used threshold of median CERES Score <-0.5028, including CDC20, NSF, ELL, and CAPZB in breast cancer cells, CCND1 for renal cell cancer cells, DHX16, HYOU1, ABCF1, PPP1R10, NOL11, BPTF, DDX39B, EXOC3, and VARS2 for lung cancer cells, CDC27, NSF, and KANSL1 for ovarian cancer cells, and LSM2, CTDP1, LSM6, ABCE1, TCP1, PFDN6, MZT1, CPSF3, DDX23, GTF2H4, MRPL45, SMG7, NOLC1, FDPS, C1QTNF4, and PRRC2A in prostate cancer cells (Supplementary Fig. 3). Here, NSF is linked to ovarian cancer.