We found through gelatine zymography assays that CM derived from the co-culture experiments (MLKL-overexpressing tumour cells and macrophages) had an improved ability to digest gelatine, but this property was reversed by MMP inhibitors, MET inhibitors and GW, primarily affecting by MMP2 and MMP9 (Supplementary Fig. 11c). Here, MLKL is linked to neoplasm.