To test whether Notch signaling regulates NE differentiation in prostate cancer, we utilized the Rosa26-loxP-STOP-loxP-Nicd1-EGFP allele that ectopically expresses the Notch1 intracellular domain (Nicd1) in mice after Cre-mediated recombination, constitutively activating Notch signaling independent of the ligand (44). The gene discussed is NOTCH1; the disease is Familial prostate cancer.