Using combinative in situ immunocytochemistry/laser-catapult-microdissection/single-cell multiplex qPCR methodology within the context of a validated in vivo experimental model for insulin-induced hypoglycemia (IIH) (Paranjape & Briski, 2005), that work showed that VMNdm Ghrh neurons express hypoglycemia-sensitive SF-1 mRNA and that Ghrh neurotransmission is critical for optimal hypoglycemic induction of counterregulatory hormone outflow. This evidence concerns the gene GHRH and pseudotumor cerebri.