When anti-MSLN CAR-T cells combined with OAd-TNFa-IL2 (an oncolytic adenovirus expressing TNF-α and IL-2) were applied to human PDA xenotransplantation mice, the activity, proliferation, tumor invasion and killing ability of the resulting anti-MSLN CAR-T cells were enhanced. This evidence concerns the gene IL2 and Patent ductus arteriosus.