Immunohistochemistry (IHC) data from the Human Protein Atlas (https://www.proteinatlas.org) demonstrated that the increased TDP‐43 protein expression exists in ESCC tissues, but not in normal esophageal tissues (Figure 1F). Furthermore, evidently elevated TDP‐43 expression in malignant tissues of ESCC patients with advanced diseases (higher cancer grades, higher AJCC stages, or more lymph node metastases) was observed compared to individuals with local diseases (lower cancer grades, lower AJCC stages, or less lymph node metastases) in TCGA (all P < 0.001) (Figure 1G–I). Here, TARDBP is linked to esophageal squamous cell carcinoma.