To investigate the feedback regulation of the Wnt/β‐catenin signaling pathway through AXIN2 in human colorectal cancer cells with mutant APC, we generated AXIN2 knockout clones in SW480 cells using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated 9 (Cas9) technology. This evidence concerns the gene AXIN2 and colorectal cancer.