Our study identified the active compounds of AOF and its important compounds and mechanism of action in the treatment of cellular senescence in DKD, and showed that AOF may delay the progression of cellular senescence in DKD by inhibiting TP53 as well as inhibiting the phosphorylation of SRC, STAT3, PIK3CA, and AKT, which provides a modern theoretical basis for the application of AOF in the treatment of DKD. This evidence concerns the gene PIK3CA and diabetic kidney disease.