We found Toll-like receptor 5 (TLR5) is positively correlated with infiltrated monocytes in both diseases; Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) is also positively associated with the neutrophils in stroke and monocytes in MS; while it is negatively associated with naive CD4 T cells and Tregs in stroke; and negatively associated with resting memory CD4 T cell in MS. Here, TLR5 is linked to stroke disorder.