We investigated the accumulation of p6232 by selective inhibition of class III PI3K (PI3K-C3, VPS34), a major component of the PI3K complex required for autophagosome formation in MA, using wortmannin (Wtn) in HeLa cells under chronic AD after three repeated treatments with U18666A and AD. The gene discussed is PIK3C3; the disease is Alzheimer disease.