Age, gender, ALT, main tumor size, multinodular, cirrhosis, TNM stage, BCLC stage, CLIP stage, tumor grade, TMB, stromal score, immune score, ESTIMATE score, risk score, CNLC stage, hepatitis B, Lymph node invasion, vascular invasion, perineural invasion, albumin, AFP, CEA and CA199. This evidence concerns the gene AFP and hepatitis B virus infection.