Multiplex fluorescent immunofluorescence of human glioma tissue further showed positive correlation of HEC1 expression in glioma with the infiltration of M2 macrophages (CD68+CD163+) (Figure 8A), the malignant degrees of gliomas (Figure 8B), and a lower survival probability of patients with high HEC1 expression (Figure 8C). Here, CD68 is linked to central nervous system cancer.