These results demonstrate that the heightened H3K27AC signal induced by ANKRD22 overexpression can, on one hand, promote the increased expression of cytoskeleton‐related proteins (PLEK2, FERMT1) and cell cycle‐related proteins (GINS3, POLE2), thereby enhancing the invasion, migration and proliferation of NPC cells. Here, ANKRD22 is linked to nasopharyngeal carcinoma.