Compared with the Control and PZH groups, the expressions of Jun, Wif1, Axin2, Ctnnb1, Wnt6, Wnt10a, Dkk2, Notum, Nkd1, Sox17, Fzd10, Wnt3 and Wnt16 were significantly up-regulated in AOM/DSS group (#P < 0.05). This evidence concerns the gene AXIN2 and infectious otitis media.