The qRT-PCR results showed that the mRNA expressions of Jun, Wif1, Axin2, Ctnnb1, Dkk2, Notum, Nkd1, Fzd10 and Wnt16 were significantly up-regulated in the AOM/DSS group compared with the Control and PZH groups, while PZH treatment significantly down-regulated these mRNA expressions (Fig. 7C). This evidence concerns the gene DKK2 and infectious otitis media.