Compared with the AOM/DSS group, the expressions of Jun, Wif1, Axin2, Ctnnb1, Wnt6, Wnt10a, Dkk2, Notum, Nkd1, Sox17, Fzd10, Wnt3, Wnt16 were observably down-regulated in PZH treatment group(*P < 0.05) (Fig. 7A-B). This evidence concerns the gene NOTUM and infectious otitis media.