To test whether lncRNA HOXA-AS3 inhibition attenuated invasive ability via regulation of miR-218-5p and FOXP1, osteosarcoma cells were co-transfected with sh-HOXA-AS3 and miR-218-5p inhibitor, or sh-HOXA-AS3 and pcDNA FOXP1. Here, FOXP1 is linked to osteosarcoma.