Briefly, the abnormally upregulated TNKS1BP1 in HCC cells inhibited the autophagy process and promoted the FAS process of HCC cells by deactivating the JAK2/STAT3 pathway, owing to the degradation of CNOT4 via K48- and K6-linked ubiquitination by TRIM21. This evidence concerns the gene STAT3 and hepatocellular carcinoma.